Fluorescence-Based Biosensing and Bioimaging
Delayed fluorescence (DF) from photosynthetic systems indicates the physiological and environmental changes. Expression of the green fluorescent protein (GFP) gene in a variety of cells and microbes makes it a useful mechanism for sensing factors affecting GFP expression and fluorescence. DF and GFP are used to develop sensors for medical, plant, bioprocessing and other applications.
Objectives & Activities:
This research consists of several components in each of the two areas. The objectives and activities include:
- Develop plant tissue-based biosensing (modeling of delayed luminescence kinetics in plant tissues, Kalman filtering, physiological state observers, and application in environmental sensing)
- Develop green fluorescent protein (GFP)-based biosensing techniques (fluorescence modeling, extended Kalman filter for time-delay systems, reaction kinetics, state observers, and application in plant cell cultures).